Cellular and Molecular Physiology

Research Summary

The patch-clamp method developed by Erwin Neher and Bert Sakmann in 1976 enabled us to record single-channel currents. This method also makes it easy to record whole-cell currents and modulate the intracellular environment. Since this method has been applied to various cells, new channels have been discovered one after another, and detailed gating and intracellular regulation mechanisms of known channels have been elucidated. On the other hand, the advance of molecular biology such as cloning and transmutation of genes, GFP, imaging, and virus vectors, enabled us to clarify the structure, function, and fate of channel proteins in various cells. Using a variety of techniques, we investigate physiological and pathophysiological functions of ion channels located on the plasma membrane. Recently, we also initiated a hippocampal research project using optogenetics and patch-clamp recording. If you study basic medicine for a term and master how to study (i.e., setting a working hypothesis, conducting experiments with appropriate methods, and writing a concise paper), you can become a good doctor. We welcome both candidates for basic medical researchers and clinicians.